Your company is growing plants that produce very valuable chemical X in roots. Genes responsible for the biosynthesis of this metabolite are Gene XA, GeneXB and GeneXC. These genes express only in roots because of the root-specific transcription factor, TFX, which regulates them.
These plants cannot be used for the commercial production of X because:
Collecting the roots will kill plants, so you need to re-grow them often;
Roots are small in sizes (root’s biomass is not big)
As a result, it is not easy to collect enough biomass from roots every year for the commercial production of X.

To increase the production of the chemical X your company decided to re-direct the production of chemical X from roots to leaves of the same plants. The rationales behind this decision are:
These plants have big leaves,
To collect leaves, you do not need to kill plants and re-grow them,
Leaves and roots have the same genome, so all genes involved in the production of X exist in leaves.
You are the lucky postdoc who has to run this project. You need to designe a strategy to conduct this project. For commercial production of the generated transgenic plants under field conditions, they have to meet modern (advanced) genetic engineering conditions.

You need to:
1. Design a transgene and explain its every component (you can design more than one transgene): 20 marks
2. Design the vector that contains your transgene: 20 marks
3. Design a strategy for the generation of the transgenic plant: 20 marks
4. Design a strategy to confirm your generated plant: 10 marks
5. Expression of genes X in leaves could change the expression profiles of other genes. How can you assess the global molecular changes in leaf cells? : 20 marks
6. Which adverse effects do you expect in your experiment: 10 marks?